EUR 75,00 USD 100.00
Kilobaser OliPure is a fast, simple and highly efficient method for oligo purification and concentration.
Use this magnetic beads based oligo purification kit after oligo synthesis or labeling applications as an alternative to HPLC oligo purification. The Kilobaser OliPure Kit contains material for 10 preps.
Following a simple bind-wash-elute principle, this oligo purification kit is based on the precipitation of DNA or RNA on magnetic beads. These beads can be immobilized in a magnetic field generated by a magnetic rack. Thus, the liquid phase can be easily removed without losing beads.
Oligonucleotides are precipitated and immobilized on the magnetic beads, all other components in the sample remain unbound. During the washing step these components are removed. Bound oligonucleotides are eluted with water or TE buffer from the magnetic beads resulting in purified and dissolved oligonucleotides.
Eluted with nuclease free water, this magnetic beads based purification kit purifies oligos from any reaction components including buffer salt, proteins and labeling components.
This chromatogram of an absorbance spectrum shows FAM+BHQ-1 DNA probes with the same sequence, synthesized with Kilobaser DNA & RNA Synthesizer unpurified (black) and purified with Kilobaser OliPure Kit based on magnetic beads (purple) as well as a HPLC purified Eurofins DNA probe with the same sequence (blue).
The result shows the equivalence of purity between the DNA probe purified with the Kilobaser OliPure kit and the DNA probe purified with HPLC from Eurofins.
Reagents for 10 oligo purifications are supplied as follows:
Note: Supplied without magnetic rack. Kilobaser Magnetic Rack must be ordered separately.
Add your resuspended oligos to the magnetic beads. Your oligos will immediately bind to the beads.
By fixing the Magnetic Beads with a magnet, you can easily wash away any contamination without losing oligos.
With nuclease-free water you can easily detach your oligos from the beads and obtain 'HPLC-pure' oligos.
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