Kilobaser Oli-Pure

Following a simple bind-wash-elute principle, this oligo purification kit is based on the precipitation of DNA or RNA on magnetic beads. These beads can be immobilized in a magnetic field generated by a magnetic rack. Thus, the liquid phase can be easily removed without losing beads.

Oligonucleotides are precipitated and immobilized on the magnetic beads, all other components in the sample remain unbound. During the washing step these components are removed. Bound oligonucleotides are eluted with water or TE buffer from the magnetic beads resulting in purified and dissolved oligonucleotides.

• Oligo purification after synthesis
• DNA purification after PCR reactions
• Purification after oligo labeling
• Purification for modified oligos
• Oligo purification after enzymatic reactions
• DNA purification for sequencing
• Concentration of oligos
• Isolating DNA from buffer

Eluted with nuclease free water, this magnetic beads based purification kit purifies oligos from any reaction components including buffer salt, proteins and labeling components.

This chromatogram of an absorbance spectrum shows FAM+BHQ-1 DNA probes with the same sequence, synthesized with Kilobaser DNA & RNA Synthesizer unpurified (black) and purified with Kilobaser OliPure Kit based on magnetic beads (purple) as well as a HPLC purified Eurofins DNA probe with the same sequence (blue).

The result shows the equivalence of purity between the DNA probe purified with the Kilobaser OliPure kit and the DNA probe purified with HPLC from Eurofins.

Reagents for 10 oligo purifications are supplied as follows:

• 10 x 50 μL Magnetic Beads Suspension
• 2 x 500 μL Binding Buffer
• 2 x 500 μL Washing Buffer
• Step-by-Step Instruction

Note: Supplied without magnetic rack. Kilobaser Magnetic Rack must be ordered separately.