For your Express DNA machine to function properly, an argon gas supply with pressure between 6 and 10 bar needs be connected to Kilobaser.
Have a look at our gas supply tutorial, where Alex shows you how to connect a 1 liter argon gas bottle to the push-in connector on the Kilobaser using a 6 mm O.D. flexible pneumatic tubing. You can either use a gas houseline or a gas bottle as you can see in the video. Gas cylinders can be obtained in several different sizes. For an idea: a 50 liter, 10 liter or 1 liter compressed argon cylinder (all 200 bar) will suffice for approximately 1000, 200 or 20 syntheses, respectively.
You can usually rent gas bottles and get refillings from your local gas supplier. We provide you with the specifications, which you send to your supplier to get the right gas bottle with the correct gas regulator.
For more detailed information about the gas supply check out our blog post: Gas Supply for Kilobaser.
For your Express DNA machine to function properly an argon gas supply with pressure between 6 and 10 bar needs be connected to Kilobaser. For that, you have two options.
One option is that you have a gas house-line. Another option is that you get a gas bottle, as you can see in the video. Gas cylinders can be obtained in several different sizes. You can choose between one liter, 10 liter, and 50 liter bottle. One liter compressed argon cylinder (all 200 bar) will suffice for approximately 1000, 200, or 20 syntheses, respectively.
On the bottles, there is a connector which consists of two pressure scales. The scale on the left shows the gas pressure and the scale on the right side is outlet pressure. For Kilobaser to work properly, you need to set the outlet pressure to six bars.
Next to pressure scales there is a connector for a tube. The 6 mm O.D. flexible pneumatic tubing comes together with the Kilobaser DNA machine. You need to put one part of the tube in the gas bottle and the other part into Kilobaser. After that, you are all set up. You can usually get the gas bottles from your local gas supplier. You can buy or rent them. After you use all the gas from the bottle, you can refill them without buying a new bottle. We will provide you with the paperwork and the specifications that you need, so you can send them to your local gas supplier and get the right gas bottle with the right pressure.