Introducing OliPure 6-FAM/BHQ-1: Targeted Purification for Dual-Labeled Probes

Precision-Engineered for Fluorescent Probe Applications

The OliPure 6-FAM/BHQ-1 kit features optimized hydrophobic interaction chromatography specifically designed for dual-labeled probes synthesized on Kilobaser ONE XT systems. Our column chemistry employs silica-based support material functionalized with C18 alkyl chains, providing selective binding through hydrophobic interactions. The 300 Å pore size has been carefully selected to ensure optimal accessibility for oligonucleotide diffusion while maintaining high binding capacity.

This targeted approach recognizes that different oligonucleotide modifications require distinct purification strategies. By focusing on 6-FAM/BHQ-1 dual-labeled probes—widely used in qPCR, FISH, and other fluorescence-based applications—we've refined our protocols to deliver superior purity and yield for these specific constructs.

Streamlined Five-Step Protocol

The purification process remains remarkably straightforward:

1. Binding phase: Crude probe dissolved in TEAA buffer (part of kit) binds to the hydrophobic resin
2. First wash: 25% acetonitrile removes unbound 6-FAM and incomplete probes
3. Salt removal: Aqueous wash eliminates residual salts and synthesis byproducts
4. Elution: 50% acetonitrile releases the purified probe
5. Dry: Use Kilobaser to dry the product

The entire process completes in under 10 minutes using standard laboratory centrifuges, with visual color changes providing immediate feedback on purification progress. The characteristic yellow eluent during the first wash confirms successful removal of excess fluorophore.

Enhanced Performance for Sensitive Applications

Our validation studies demonstrate that OliPure 6-FAM/BHQ-1 effectively removes the primary contaminants that interfere with fluorescence-based assays: unbound 6-FAM dye, salt residues from synthesis, and incomplete probes. This results in significantly reduced background fluorescence and improved signal-to-noise ratios in downstream applications.

The kit includes pre-measured solvents optimized for 6-FAM/BHQ-1 chemistry, eliminating buffer preparation and ensuring consistent results. Each kit provides materials for five purifications, with columns designed to handle the typical synthesis scales produced by Kilobaser ONE XT.

Looking Forward: Application-Specific Solutions

The OliPure 6-FAM/BHQ-1 kit represents our commitment to providing researchers with tools precisely matched to their applications. Rather than offering one-size-fits-all solutions, we're developing a portfolio of targeted purification kits that recognize the unique chemistry of different oligonucleotide modifications.

This approach allows us to optimize every aspect of the purification process—from resin chemistry to elution conditions—for specific probe types. Researchers benefit from improved purity, higher yields, and simplified protocols tailored to their exact needs.

Integration with Your Synthesis Workflow

For laboratories using Kilobaser ONE XT for probe synthesis, the OliPure 6-FAM/BHQ-1 kit provides the essential link between synthesis and application. The seamless integration enables complete in-house probe production, from design to purified product, in less than three hours total workflow time.

We've included a straightforward quantification protocol using standard microvolume spectrophotometry, allowing immediate assessment of probe concentration and purity. Our support team remains available to assist with protocol optimization for specific applications or to discuss purification strategies for other probe chemistries.

The OliPure 6-FAM/BHQ-1 kit is now available for order. Visit our product page for detailed specifications and ordering information. We look forward to supporting your fluorescent probe applications with this targeted purification solution.

Stay tuned for announcements about additional application-specific OliPure kits currently in development. Your feedback on specific purification challenges helps guide our product development priorities.