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Reporter Modification

6-FAM Fluorescent dye

Whether DNA or RNA, 6-FAM is used to label biomolecules. This green fluorescent dye is the most popular reporter dye for DNA probes.

With Kilobaser one-XT, you have unlimited access to customized fluorescent probes for your projects at any time.

Start your flourescent probe synthesis right away

with Kilobaser DNA & RNA synthesizer

TaqMan probes easily synthesized with Kilobaser personal DNA Synthesizer with reagent cartridge and microfluidic chip
FISH Probe_Kilobaser_Supply-Combination

Just 2 consumables and 3 easy steps:

Synthesize TaqMan or Molecular Beacon probes with our 6-FAM cartridge for reporter labeling in combination with our BHQ-1 chip for quencher labeling.

Use the 6-FAM cartridge in combination with the FISH chip to synthesize FISH probes.

Fluorescent reporter dye labeling
with Kilobaser

Ready-to-use probes in 3 steps

enter sequence

Enter your DNA sequence using the included touch screen, USB flash drive or your computer.

insert chip & cartridge

Insert Kilobasers' patented microfluidic chip & reagent cartridge for DNA probe synthesis.

Press start

Within two hours you get your customized fluorescent DNA probe!

You want to learn more about synthesis with Kilobaser?

About 6-FAM Fluorescent dye

TaqMan probe binding to target DNA. Polymerase hydrolizing reporter to seperate it from DNA strand. Reporter signal is no longer absorbed and can now be detected.
Molecular Beacon probe binding to target DNA. This seperates Reporter an quencher to a distance, so the emitted reporter signal is no longer absorbed from quencher.

the principle:

6-FAM  reacts with amines at its carboxy group and can only be released by hydrolysis. This covalent bond makes the fluorescent dye so interesting for biochemistry. Thanks to its reactivity with amines, it can be easily incorporated into DNA probe synthesis.

As a reporter on DNA probes, 6-FAM is added to the 5′-end of the DNA. Since DNA synthesis proceeds from the 3′-end to the 5′-end, the dye is attached at the end of DNA/RNA synthesis.

The most common use of fluorescent labeled probes is for diagnostic applications in research and medicine, for example in multiplex PCR. To synthesize dual labeled probes, a quencher is additionally attached to the 3′-end. 

The high specificity of complementary DNA binding allows to detect any DNA sequence within a biological sample. The fluorescent dye is also recommended for quantity analysis, such as qPCR and rtPCR.

6-FAM DNA probes with Kilobaser

6-FAM Modification

Our 6-FAM reagent cartridge contains all the required synthesis reagents for 5'-end labeled DNA probes. The reagents are safely contained in the cartridge, so you are is no risk of getting in contact with the hazardous reagents.
Kilobaser cartridges allow 5'-end modification.
One 6-FAM cartridge allows 100 bases to be synthesised.

Combination & Consumption
  • The 6-FAM cartridges and Microfluidic Fluidic Chips can be used to produce one or more DNA probes labeled at the 5’-end 6-FAM with a total of up to 100 bases, also known as FISH (fluorescence in situ hybridization) probes.
  • Combining the 6-FAM cartridge and BHQ-1 microfluidic chips for synthesis, one or more TaqMan DNA probes with a total of up to 100 bases can be produced.

You have further questions? Visit our FAQ-section.

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Lisa-Marie Michelitsch​

Master of Science
Biotechnology

[email protected]
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