Whether DNA or RNA, 6-FAM (Fluorescein labeled amidite) is used to label biomolecules. This green fluorescent dye is the most popular reporter dye for DNA probes. 6-FAM reacts with amines at its carboxy group and can only be released by hydrolysis.
This covalent bond makes the fluorescent dye so interesting for biochemistry. Thanks to its reactivity with amines, it can be easily incorporated into DNA probe synthesis.
As a reporter on DNA probes, 6-FAM is added to the 5′-end of the DNA. Since DNA synthesis proceeds from the 3′-end to the 5′-end, the dye is attached at the end of DNA/RNA synthesis.
The most common use of fluorescent labeled probes is for diagnostic applications in research and medicine, for example in multiplex PCR. To synthesize dual labeled probes, a quencher is additionally attached to the 3′-end.
The high specificity of complementary DNA binding allows to detect any DNA sequence within a biological sample. The fluorescent dye is also recommended for quantity analysis, such as qPCR and rtPCR.
Our 6-FAM reagent cartridge contains all the required synthesis reagents for 5'-end labeled DNA probes. The reagents are safely contained in the cartridge, so you are is no risk of getting in contact with the hazardous reagents.
Kilobaser cartridges allow 5'-end modification.
One 6-FAM cartridge allows 100 bases to be synthesised.
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