The Synthesis Platform consists of the actual synthesis device Kilobaser and two consumables - DNA Cartridges & Fluidic Chips.
For operation, both consumables must be inserted into the device.
A DNA cartridge contains all necessary synthesis reagents (especially the 4 base building blocks for adenine, guanine, cytosine, thymine in the form of phosphoramidites) for a total of 150 bases.
The instrument uses argon gas pressure to transfer the reagent required for each synthesis step from the cartridge into the Fluidic Chip. The Fluidic Chip has two main functions:
- The tiny fluid valves on it control the flow of reagents;
- In the micro-synthesis chamber (classically known as ‘columns’), the actual solid- phase synthesis of the DNA oligos takes place. To synthesize a DNA oligo with 20 bases, Kilobaser needs less than 1.5 hours, and the yield is around 300 picomoles.