fish probes

this fish is not just a fish

Oligonucleotide probes for fluorescent in situ hybridization (FISH) are used for specific detection of DNA and RNA sequences. Whether in  tissues, cells, cell compartments or chromosomes they highlight their target sequence.
FISH probes are injected into living cells (in situ), where they find their target with highest precision and bind to it (Hybridization).

FISH Probe application

Depending on whether DNA oligos or RNA oligos are fluorescently labeled, different applications arise.

DNA FISH probes

Gene (mutation) detection

Chromosomal gene localization

Genetic analysis

Microbial detection

RNA FISH probes

Transcript sequence detection

Gene expression analysis

Kilobaser DNA Synthesizer machine for in-house DNA Synthesis. Microfluidic chip based. Synthesize DNA oligos, DNA primer, DNA probes in less than 2h. 6-FAM cartridge fo benchtop DNA Synthesizer.

Direct sequence detection with 6-FAM labeled probes

With the 6-FAM reagent cartridge for Kilobaser you can easily synthesize your custom FISH probes in no time.

6-Fam Facts:

Absorption - 495 nm

Emission - 517 nm

5'-end modification

FISH probe microfluidic chip combined wit 6-FAM fluorescent dye reagent cartridge. Kilobaser in-house benchtop DNA synthesizer.

Synthesize fish probes on your lab bench!

Combine Kilobaser 6-FAM reagent cartridge with Kilobaser FISH microfluidic chip to synthesize labeled FISH probes with a length of up to 50nt!

FISH Chip + 6-FAM Cartridge

FAQ – FISH probes

Where are the FISH probes fluorescence labeled?

FISH probes synthesized by Kilobaser are labeled with 6-FAM fluorescent dye at the 5'-end .

Are the FISH probes ready to use?

Yes! With the Kilobaser you will get crude dryed FISH probes. Since the synthesis requires extremely few reagents, the by-products in your synthesis are minimal.

With in-situ hybridization, you do not need to clean your probe separately, as washing steps are included in this application.

If you still want to clean your probes, you can easily do it yourself with our purification kit TrashAway.

Can I also synthesize RNA FISH probes?

YES, you can!

With Kilobaser you are able to synthesize fully 2'MOE modified RNA or fully phosphorothioated RNA labeled on 5'-end with 6-FAM fluorescent dye.

You have further questions? Maybe you will find the answer in our FAQ-section.

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We are happy to help you

Lisa-Marie Michelitsch‚Äč

Master of Science
Biotechnology

sales@kilobaser.com
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