The all-rounder among DNA probes, the TaqMan probe is mainly used for the detection of specific sequences.
In combination with a PCR method, it can be used in a variety of ways.
Whether DNA, RNA or proteins, there are almost no limits to using the TaqMan probe.
With Kilobaser Personal Synthesizer, you have unlimited access to customized TaqMan probes for your projects at any time.
Enter your DNA sequence using the included touch screen, USB flash drive or your computer.
Insert Kilobasers' patented microfluidic chip & reagent cartridge for DNA probe synthesis.
Within two hours you get your customized TaqMan probe!
TaqMan probes are short DNA sequences with a fluorescent label at each end. The 5‘-end modifier serves as a reporter and is excited to fluorescence by a specific wavelength. The quencher, the modification at the 3‘-end, absorbs the emitted light from the reporter as long as it is in close proximity.
During PCR extension, the primer is cleaved nucleotide by nucleotide from the target DNA by a 5′ exonuclease activity of the TaqMan probe. Thus, the reporter is also cleaved from the DNA and moves further away from the quencher. Now, the quencher can no longer absorb the emitted signal. This results in an increasing signal.
Your cartridge contains enough reagents for 100 bases. The number of TaqMan probes you can synthesize depends on the length of your sequence.
Sequence length: 25 bases
Number of probes: 4
First of all you need a Kilobaser desktop synthesizer.
With this you can not only synthesize TaqMan probes, but also standard DNA oligos, FISH probes and even RNA.
You also need our patented 6-FAM DNA cartridge, which contains all the reagents you need for your synthesis.
Finally, you will need one of our patented BHQ-1 chips per TaqMan sample synthesis. The BHQ-1 quencher for your synthesis is bonded to this chip.
Yes, of course you can test the TaqMan probes beforehand.
Just send us your desired sequence using the linked form.
If you want to learn more about Kilobaser personal synthesizers in the meantime, just book your exclusive online demo and we will answer all your questions!
We are looking forward to it!