TaqMan probes synthesized with Kilobaser personal DNA & RNA Synthesizer.

Taqman probes

Dual labeled with 6-FAM and BHQ-1

The all-rounder among DNA probes, the TaqMan probe is mainly used for the detection of specific sequences.
In combination with a PCR method, it can be used in a variety of ways.
Whether DNA, RNA or proteins, there are almost no limits to using the TaqMan probe.

With Kilobaser Personal Synthesizer, you have unlimited access to customized TaqMan probes for your projects at any time.

TaqMan probes synthesized with Kilobaser personal DNA & RNA Synthesizer.

Start your TaqMan probe synthesis right away

with Kilobaser personal synthesizer

TaqMan Probe_Kilobaser_Supply-Combination

Just 2 consumables and 3 easy steps:

Label your TaqMan probe with our 6-FAM cartridge at the 5′ end with a 6-FAM reporter. With one 6-FAM cartridge you can synthesize up to 100 nucleotides!

Use one BHQ-1 chip per synthesis to label your TaqMan probe on the 3′ end with a BHQ-1 quencher.

TaqMan Probe Synthesis with Kilobaser

Ready-to-use TaqMan probes in 3 steps

enter sequence

Enter your DNA sequence using the included touch screen, USB flash drive or your computer.

insert chip & cartridge

Insert Kilobasers' patented microfluidic chip & reagent cartridge for DNA probe synthesis.

Press start

Within two hours you get your customized TaqMan probe!

You want to learn more about synthesis with Kilobaser personal synthesizer?

About TaqMan Probes

TaqMan probe annealing with target Sequence. Kilobaser TaqMan probes.
TaqMan probe digestion by elongation during PCR with TaqMan Polymerase.

possible methods & Applications:

Real-Time/qPCR

RT-qPCR

Pathogen detection

Genotyping assay

Gene expression assay

MicroRNA assay

Mutation detection

Protein quantification

TaqMan probes are short DNA sequences with a fluorescent label at each end. The 5‘-end modifier serves as a reporter and is excited to fluorescence by a specific wavelength. The quencher, the modification at the 3‘-end, absorbs the emitted light from the reporter as long as it is in close proximity.

During PCR extension, the primer is cleaved nucleotide by nucleotide from the target DNA by a 5′ exonuclease activity of the TaqMan probe. Thus, the reporter is also cleaved from the DNA and moves further away from the quencher. Now, the quencher can no longer absorb the emitted signal. This results in an increasing signal.

FAQ – TaqMan Probes

How many TaqMan probes can I synthesize with one cartridge?

Your cartridge contains enough reagents for 100 bases. The number of TaqMan probes you can synthesize depends on the length of your sequence.

For example:

Sequence length: 25 bases
Number of probes: 4

What exactly do I need to synthesize my TaqMan probe?

First of all you need a Kilobaser desktop synthesizer.
With this you can not only synthesize TaqMan probes, but also standard DNA oligos, FISH probes and even RNA.

You also need our patented 6-FAM DNA cartridge, which contains all the reagents you need for your synthesis.

Finally, you will need one of our patented BHQ-1 chips per TaqMan sample synthesis. The BHQ-1 quencher for your synthesis is bonded to this chip.

Can I test the TaqMan probes before ordering a Kilobaser desktop synthesizer?

Yes, of course you can test the TaqMan probes beforehand.
Just send us your desired sequence using the linked form.
If you want to learn more about Kilobaser personal synthesizers in the meantime, just book your exclusive online demo and we will answer all your questions!
We are looking forward to it!

You have further questions? Visit our FAQ-section.

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We are happy to help you

Lisa-Marie Michelitsch​

Master of Science
Biotechnology

[email protected]
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