Quencher

BHQ-1 Probes

DNA probe Quencher BHQ-1 absorbs wavelenght between 480-580 nm.

BHQ-1 quencher for DNA probes is known for its large absorption range. Black Hole Quencher-1 is an excellent partner for many reporter dyes. Since it does not fluorescence itself, very little background noise is produced. This makes signal detection of the reporter even more accurate.

So-called FRET (fluorescence resonance energy transfer) probes are based on the principle that the quencher, in the close proximity of a reporter, absorbs emittied wavelength. At this point, reporter signal is absorbed in large part. Only when the reporter moves away from the quencher its emission can be fully detected.

Usually, a quencher is synthesized at the 3′-end of a DNA sequence. Thanks to its biochemical properties, the modification can be included in the normal process of DNA synthesis.

On our BHQ-1 microfluidic chip  for the Kilobaser, the BHQ-1 is anchored to the CPG material of the microcolumn. This ensures that the synthesis has a quencher at the 3′-end as desired. In combination with a suitable reporter, such as the 6-FAM fluorescent dye at the 5′-end of the DNA probe, many applications become possible.

Microfluidic Chip with integrated CPG column, modified with BHQ1- Quencher dye, for DNA probe synthesis

With the BHQ-1 microfluidic chip for Kilobaser, you can easily and quickly synthesize your own DNA probes.

BHQ-1 Facts:

Absorption 480 nm - 580 nm

No Emission

3'-end modification

Kilobaser Supply combinations for DNA probe synthesis.

DNA Probe Types –
Supply Combinations

For even more applications, combine the BHQ-1 chip with our 6-FAM cartridge!

BHQ-1 Chip + 6-FAM Cartridge

Standard Chip + 6-FAM Cartridge

BHQ-1 Probes with Kilobaser

BHQ-1 Modification

The microfluidic chip with integrated micro column is the solid phase where the DNA synthesis starts and the modification is anchored. Kilobaser microfluidic chips allow 3'-end modification such as BHQ-1. The optimized pressure valve system forms the basis of the Kilobaser microfluidc chip technology.
The chip can be used only once.

Combination & Consumption
  • The 6-FAM cartridges and Microfluidic Fluidic Chips can be used to produce one or more DNA probes labeled at the 5’-end 6-FAM with a total of up to 100 bases, also known as FISH (fluorescence in situ hybridization) probes.
  • Using the BHQ-1 MicroFluidic Chips and a standard DNA cartridge, multiple DNA primers labeled at the 3’-end BHQ-1 can be prepared with a total of up to 150 bases.
  • Combining the 6-FAM probe cartridge and BHQ-1 Fluidic Chips for synthesis, TaqMan DNA probes with a total of up to 100 bases can be produced.

You have further questions? Maybe you will find the answer in our FAQ-section.

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We are happy to help you

Lisa-Marie Michelitsch​

Master of Science
Biotechnology

sales@kilobaser.com
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