BHQ-1 quencher for DNA probes is known for its large absorption range. Black Hole Quencher-1 is an excellent partner for many reporter dyes. Since it does not fluorescence itself, very little background noise is produced. This makes signal detection of the reporter even more accurate.
So-called FRET (fluorescence resonance energy transfer) probes are based on the principle that the quencher, in the close proximity of a reporter, absorbs emittied wavelength. At this point, reporter signal is absorbed in large part. Only when the reporter moves away from the quencher its emission can be fully detected.
Usually, a quencher is synthesized at the 3′-end of a DNA sequence. Thanks to its biochemical properties, the modification can be included in the normal process of DNA synthesis.
On our BHQ-1 microfluidic chip for the Kilobaser, the BHQ-1 is anchored to the CPG material of the microcolumn. This ensures that the synthesis has a quencher at the 3′-end as desired. In combination with a suitable reporter, such as the 6-FAM fluorescent dye at the 5′-end of the DNA probe, many applications become possible.
For even more applications, combine the BHQ-1 chip with our 6-FAM cartridge!
The microfluidic chip with integrated micro column is the solid phase where the DNA synthesis starts and the modification is anchored. Kilobaser microfluidic chips allow 3'-end modification such as BHQ-1. The optimized pressure valve system forms the basis of the Kilobaser microfluidc chip technology.
The chip can be used only once.
You have further questions? Maybe you will find the answer in our FAQ-section.
* required fields