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Quencher Modification

BHQ-1

Black Hole Quencher 1 is known for its large absorption range from 480nm – 580nm. That is why BHQ-1 is so popular as quencher modification on dual labeled DNA probes.

With Kilobaser DNA & RNA Synthesizer Extended Edition, you have unlimited access to custom modified DNA and RNA for your projects at any time.

Start your DNA probe synthesis right away

with Kilobaser personal synthesizer

TaqMan probes easily synthesized with Kilobaser personal DNA Synthesizer with reagent cartridge and microfluidic chip
Molecular Beacon DNA probes. Synthesize BHQ-1 quencher labeled DNA probes with Kilobaser desktop DNA synthesizer

Just 2 consumables and 3 easy steps:

Synthesize TaqMan or Molecular Beacon probes with our 6-FAM cartridge for reporter labeling in combination with our BHQ-1 chip for quencher labeling.

Quencher labeling
with Kilobaser

Ready-to-use probes in 3 steps

enter sequence

Enter your DNA sequence using the included touch screen, USB flash drive or your computer.

insert chip & cartridge

Insert Kilobasers' patented microfluidic chip & reagent cartridge for DNA probe synthesis.

Press start

Within two hours you get your customized DNA probe!

You want to learn more about synthesis with Kilobaser DNA & RNA Synthesizer Extended Edition?

About Black Hole Quencher 

DNA probe Reporter not emitting in near distance to DNA probe Quencher. DNA probe Reporter emitting in far distance to DNA probe Quencher.

the principle:

Since BHQ-1 absorbs wavelengths from 480 to 580nm and has no fluorescent property itself. As a result, very little background noise is generated. Therefore, it makes the signal detection of a corresponding reporter very accurate.

So-called FRET (fluorescence resonance energy transfer) probes are based on the principle of emitting reporter and absorbing quencher in proximity. Only when there is distance between reporter and quencher, the emission can be fully detected.

Usually, a quencher is added at the 3′-end of a DNA sequence. On Kilobaser BHQ-1 chip, the BHQ-1 is anchored to the CPG material of the microcolumn. This ensures every synthesis starts with the quencher modification. In combination with a suitable reporter, such as 6-FAM fluorescent dye at the 5′-end of the DNA probe, many applications become possible.

BHQ-1 Probes with Kilobaser

BHQ-1 Modification

BHQ-1 quencher modification is anchored to the column on the microfluidic chip. Each oligo synthesized with the BHQ-1 quencher chip is modified with the BHQ-1 quencher at its 3' end.

Combination & Consumption

Combine BHQ-1 quencher chip with 6-FAM probe cartridge to synthesize your custom TaqMan probes.

You have further questions? Visit our FAQ-section.

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Lisa-Marie Michelitsch​

Master of Science
Biotechnology

[email protected]
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