OliPure HIC Purification Kit - Supply
HIC column-based oligonucleotide purification
Purification in under 10 minutes
For sample volumes between 20 and 120 μL
For DNA/RNA oligonucleotides ≥ 12 nt in length
High recovery rate of ~75%
10 purification tubes per kit
Details – OliPure HIC Purification Kit
HIC (Hydrophobic Interaction Chromatography) uses phenyl-modified agarose to retain hydrophobic and small molecules such as unbound dyes and salt, while DNA/RNA oligonucleotides pass through immediately. The HIC column is housed in a micro-spin tube for purification by centrifugation. Since DNA/RNA oligonucleotides are unaffected by the column, purified DNA/RNA is obtained in a single spin-down.
Purification of probes after synthesis
Desalting of oligonucleotides after synthesis
Purification of probes after fluorescent labeling
Removal of phenol after phenol-chloroform extraction
Probes at the same concentration but with different degrees of purification show different absorbance spectra due to unbound dye contamination. For 6-FAM and BHQ-1-labeled DNA probes, absorbance at 495 nm varies with the amount of unbound 6-FAM.
The unpurified probe shows the highest signal at 495 nm, indicating unbound dye from synthesis. Purification with the OliPure HIC Kit reduces unbound 6-FAM to the same low levels seen in HPLC-purified probes.
Absorbance spectra of three double-labeled (6-FAM + BHQ-1) DNA probes at the same concentration, purified differently: synthesized with Kilobaser One-XT unpurified (black), purified with Kilobaser OliPure HIC Kit (purple), and HPLC-purified by a service provider (blue).
10 × OliPure HIC tubes for purification of 10 samples
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