OliPure HIC Purification Kit - Supply
HIC column based oligonucleotide purification
Purification in less than 10 minutes
For sample volume between 20 and 120μL
For DNA/RNA oligonucleotides with a length of ≥ 12nt
High recovery rate of ~75%
10 purification tubes in one kit
Details – OliPure HIC Purification Kit
HIC stands for Hydrophobic Interaction Chromatography, which is achieved by using phenyl-modified agarose as the column. The features of this column ensure that hydrophobic and small molecules such as unbound dyes and salt are retained, while DNA/RNA oligonucleotides leave the column immediately.
This HIC column is placed in a micro-spin tube to allow purification by centrifugation. Since DNA/RNA oligonucleotides are not affected by the column, purified DNA/RNA can be obtained in a single spin-down.
Purification of probes after synthesis
Desalting of oligonucleotides after synthesis
Purification of probes after fluorescent labeling
Removal of phenol after phenol-chloroform extraction
Probes of the same concentration but with different degrees of purification will have different absorbance spectra due to the degree of unbound dye contamination. For 6-FAM and BHQ-1 labeled DNA probes, absorbance at 495nm varies depending on the amount of unbound 6-FAM.
Accordingly, the unpurified probe shows the highest signal at 495nm, indicating unbound dye from oligonucleotide synthesis. Purification of the probe using the OliPure HIC Kit reduces unbound 6-FAM to the same low levels seen in HPLC-purified probes.
Absorbance spectra of three double-labeled (6-FAM+BHQ-1) DNA probes at the same concentration, but purified in different ways: probe synthesized with Kilobaser one-XT unpurified (black) and purified with Kilobaser OliPure HIC kit (purple) and a HPLC purified probe from a service provider (blue).
10 x OliPure HIC tubes for purification of 10 samples
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