- No products in the cart.
USD 110,00 excl. taxesHIC oligo purification
Please select your country to get details about prices and distributors near you.
HIC stands for Hydrophobic Interaction Chromatography, which is achieved by using phenyl-modified agarose as the column. The features of this column ensure that hydrophobic and small molecules such as unbound dyes and salt are retained, while DNA/RNA oligonucleotides leave the column immediately.
This HIC column is placed in a micro-spin tube to allow purification by centrifugation. Since DNA/RNA oligonucleotides are not affected by the column, purified DNA/RNA can be obtained in a single spin-down.
Probes of the same concentration but with different degrees of purification will have different absorbance spectra due to the degree of unbound dye contamination. For 6-FAM and BHQ-1 labeled DNA probes, absorbance at 495nm varies depending on the amount of unbound 6-FAM.
Accordingly, the unpurified probe shows the highest signal at 495nm, indicating unbound dye from oligonucleotide synthesis. Purification of the probe using the OliPure HIC Kit reduces unbound 6-FAM to the same low levels seen in HPLC-purified probes.
Absorbance spectra of three double-labeled (6-FAM+BHQ-1) DNA probes at the same concentration, but purified in different ways: probe synthesized with Kilobaser one-XT unpurified (black) and purified with Kilobaser OliPure HIC kit (purple) and a HPLC purified probe from a service provider (blue).
* required fields